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But, in the Next Generation Sequencing world, there is a whole lot more. When you are starting to think about your next sequencing experiment, people may ask if you are doing WES, WGS, or target-seq? Some other acronyms may be terms related to variant and mutation analysis. After you perform your sequencing run, there may be questions about how your sequencing results turned out.
If you need to know, AQ refers to Alignment Quality, in other words, how well did the sequence of your sample align with the reference sequence. In NGS, because you generate millions of reads or fragments of DNA, the length of the reads can vary in size, hence there is an average or mean length of the read.
The more ISPs you have the more reads can be sequenced. If these terms make your head spin, wait until you hear the terms used by your friendly bioinformatics scientist. Allow me to try to explain this language. Ok, Got it? There you have it.
The basic glossary of acronyms and terms we commonly use in NGS talk. If you have more questions on NGS, Submit them at thermofisher. Why not generating fastq files? Same information than ubam but smaller size, could be compressed, standard format and could be processed by the vast majority of ngs softwares….
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